Effect of Physiological Concentrations of Vitamin C on the Inhibitation of Hydroxyl Radical Induced Light Emission from Fe2+-EGTA-H2O2 and Fe3+-EGTA-H2O2 Systems In Vitro

Ascorbic acid (AA) has antioxidant properties. However, in the presence of Fe2+/Fe3+ ions and H2O2, it may behave as a pro-oxidant by accelerating enhancing formation hydroxyl radicals (•OH). Therefore, this study we evaluated effect AA at concentrations 1 to 200 µmol/L on •OH-induced light emission (at pH 7.4 temperature 37 °C) from 92.6 Fe2+—185.2 EGTA (ethylene glycol-bis (β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid)—2.6 mmol/L Fe3+—185.2 EGTA—2.6 H2O2 systems. Dehydroascorbic (DHAA) same range served reference compound. Light was measured with multitube luminometer (AutoLumat Plus LB 953) for 120 s after automatic injection H2O2. 50 75 completely inhibited Fe2+-EGTA-H2O2 Fe3+-EGTA-H2O2, respectively. Concentrations 100 did not affect chemiluminescence Fe3+-EGTA-H2O2 but tended increase Fe2+-EGTA-H2O2. DHAA had no both These results indicate that physiological exhibits strong activity chelated iron